ExoLiver mimics the human liver microenvironment through the combination of co-culture of primary human liver cells, constant stimulation of the endothelial cell layer with microfluidic shear stress, and continuous paracrine interactions between all cell types. Due to its cutting-edge characteristics, ExoLiver has extremely high versatility allowing its use as experimental model to deeply characterize liver diseases pathophysiology, to understand the mechanisms of action of new chemical entities, or to investigate the possible hepatotoxicity of novel compounds.